The present study was conducted to evaluate the performance of glutathione and ascorbic acid antagonism of iron-induced changes an the hematological findings, RBC fragility, Phospholipid in red blood cell membranes and serum K^+ levels in vitro. Blood was collected from vena cava of 20 Sprague-Dawley male rats(10 weeks), using 0.5 ㎎ of heparin per 10 ㎖ of blood as anticoagulant. These blood were mixed with Fe(OH)₃ for oxidation and glutathione and ascorbic acid for antioxidation.
The results obtained were summarized as follows ;
1. RBC counts of glutathione or asrcobic acid mixed groups were significantly decreased than that of negative control group, however, Significantly recovered than that of positive control group.
2. RBC fragility rates and K^+ levels of glutathione or ascorbic acid mixed groups were significantly increased than those of negative control group, however, significantly recovered than that of positive control group.
3. Phospholipid and vitamin E concentrations in the RBC Membranes of glutathione or ascorbic acid mixed groups were significantly recovered than those of positive control group.
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